trpv1 (heat receptor)  (Thermo Fisher)

Journal: International Journal of Molecular Sciences

Article Title: Thermosensitive TRP Channels Are Functionally Expressed and Influence the Lipogenesis in Human Meibomian Gland Cells

doi: 10.3390/ijms25074043

Figure Lengend Snippet: Immunofluorescence results show the expression of TRPV1, TRPV3, TRPV4 and TRPM8 in hMGECs. ( a ) Proliferating hMGECs, ( b ) differentiated hMGECs. The fluorescence microscopic images show an overlay of both the respective antibody (green) and DAPI (blue) signal. All microscopic images are presented in ×600 magnification.

Article Snippet: All six of these temperature-sensitive TRPs (thermo-TRPs) have been identified on the ocular surface: the heat receptors TRPV1, -2, -3 and -4, as well as the cold receptors TRPM8 and TRPA1 [ , , ].

Techniques: Immunofluorescence, Expressing, Fluorescence

Journal: International Journal of Molecular Sciences

Article Title: Thermosensitive TRP Channels Are Functionally Expressed and Influence the Lipogenesis in Human Meibomian Gland Cells

doi: 10.3390/ijms25074043

Figure Lengend Snippet: Immunofluorescence results show the expression of TRPV1, TRPV3, TRPV4 and TRPM8 in ( a ) murine and ( b ) human meibomian glands. The fluorescence microscopic images show an overlay of both the respective antibody (green) and DAPI (blue) signal. All microscopic images are presented in ×600 magnification.

Article Snippet: All six of these temperature-sensitive TRPs (thermo-TRPs) have been identified on the ocular surface: the heat receptors TRPV1, -2, -3 and -4, as well as the cold receptors TRPM8 and TRPA1 [ , , ].

Techniques: Immunofluorescence, Expressing, Fluorescence

Journal: International Journal of Molecular Sciences

Article Title: Thermosensitive TRP Channels Are Functionally Expressed and Influence the Lipogenesis in Human Meibomian Gland Cells

doi: 10.3390/ijms25074043

Figure Lengend Snippet: CAP or menthol increased intracellular Ca 2+ [Ca 2+ ] i in fura-2-loaded hMGECs. Time-dependent Ca 2+ changes are shown as fluorescence ratios (f340/f380), which are proportional to the [Ca 2+ ] i levels in hMGECs. Data are represented as mean ± SEM. n indicates the number of cells measured in this set of experiments. Dashed line represents the reference line for baseline value (0.1). Arrows mark the time (240 s) at which the agonist was added. ( a ) Application of 20 µM CAP increased [Ca 2+ ] i (filled circles; ( n = 15). For the control without CAP application, no changes in the [Ca 2+ ] i level could be observed ( n = 76) (open circles). ( b ) The effect of CAP could be suppressed in the presence of the specific TRPV1 blocker AMG9810 (10 µM; n = 37). ( c ) Summary of the experiments with CAP and AMG9810. Bars represent mean values ± SEM of the fluorescence ratio at 200 (control), 450 and 600 s (CAP). The numbers of cells measured in both experiments are indicated in brackets above the bars. The asterisks (*) indicate statistically significant differences with and without CAP ( n = 15 to n = 37; *** p < 0.001, ** p < 0.01, * p < 0.05; paired tested). The hashtags (#) refer to unpaired data with and without AMG9810 (### p < 0.001). ( d – f ): Same experiments as shown in ( a – c ). Instead of CAP 1, mM menthol was used (filled circles; n = 37) and instead of 10 µM AMG9810, 10 µM of TRPM8 blocker AMTB was added ( n = 46).

Article Snippet: All six of these temperature-sensitive TRPs (thermo-TRPs) have been identified on the ocular surface: the heat receptors TRPV1, -2, -3 and -4, as well as the cold receptors TRPM8 and TRPA1 [ , , ].

Techniques: Fluorescence, Control

Journal: International Journal of Molecular Sciences

Article Title: Thermosensitive TRP Channels Are Functionally Expressed and Influence the Lipogenesis in Human Meibomian Gland Cells

doi: 10.3390/ijms25074043

Figure Lengend Snippet: Results of oil red O staining (ORO) after TRPV1 and TRPM8 stimulation in proliferating and differentiated hMGECs. hMGECs were stimulated for 24 h with CAP (10 µM) or icilin (I, 100 µM). The lipid accumulation in the cells was stained with ORO, documented photographically and analyzed using ImageJ software (version 1.54 h). The bars represent the ratio of the lipid accumulation compared to untreated controls. ( a ) In the proliferating hMGECs, CAP and icilin showed no significant impact on lipid accumulation after 24 h. ( b ) In the differentiated hMGECs, there was a significant increase in lipid content after TRPV1 activation and a decrease after TRPM8 activation. ( c ) Representative images of the experiments summarized in ( a , b ). The pictures show the stained proliferating and differentiated hMGECs in ×600 magnification, the bright field image, the ORO staining (red) and an overlay of both pictures. Asterisks (* p < 0.05, **** p < 0.0001; unpaired, ordinary one-way ANOVA test indicates statistically significant results for the stimulation of TRPV1 and TRPM8).

Article Snippet: All six of these temperature-sensitive TRPs (thermo-TRPs) have been identified on the ocular surface: the heat receptors TRPV1, -2, -3 and -4, as well as the cold receptors TRPM8 and TRPA1 [ , , ].

Techniques: Staining, Software, Activation Assay

Journal: International Journal of Molecular Sciences

Article Title: Thermosensitive TRP Channels Are Functionally Expressed and Influence the Lipogenesis in Human Meibomian Gland Cells

doi: 10.3390/ijms25074043

Figure Lengend Snippet: Lipid accumulation in hMGECs after TRPV1 and TRPM8 stimulation. Immortalized hMGECs were stimulated for 24 h with CAP (10 µM) or icilin (I, 100 µM) and lipid content was analyzed and compared to unstimulated controls using ORO staining.

Article Snippet: All six of these temperature-sensitive TRPs (thermo-TRPs) have been identified on the ocular surface: the heat receptors TRPV1, -2, -3 and -4, as well as the cold receptors TRPM8 and TRPA1 [ , , ].

Techniques: Staining, Control

Journal: International Journal of Molecular Sciences

Article Title: Thermosensitive TRP Channels Are Functionally Expressed and Influence the Lipogenesis in Human Meibomian Gland Cells

doi: 10.3390/ijms25074043

Figure Lengend Snippet: Primers used for Reverse Transcriptase Polymerase Chain Reaction and quantitative Real-Time Polymerase Chain Reaction.

Article Snippet: All six of these temperature-sensitive TRPs (thermo-TRPs) have been identified on the ocular surface: the heat receptors TRPV1, -2, -3 and -4, as well as the cold receptors TRPM8 and TRPA1 [ , , ].

Techniques: Reverse Transcription, Polymerase Chain Reaction

Journal: International Journal of Molecular Sciences

Article Title: Thermosensitive TRP Channels Are Functionally Expressed and Influence the Lipogenesis in Human Meibomian Gland Cells

doi: 10.3390/ijms25074043

Figure Lengend Snippet: Antibodies used for Immunofluorescence (IF) and Western blot (WB).

Article Snippet: All six of these temperature-sensitive TRPs (thermo-TRPs) have been identified on the ocular surface: the heat receptors TRPV1, -2, -3 and -4, as well as the cold receptors TRPM8 and TRPA1 [ , , ].

Techniques: Immunofluorescence, Western Blot

Journal: BMC Neurology

Article Title: Use of the novel contact heat evoked potential stimulator (CHEPS) for the assessment of small fibre neuropathy: correlations with skin flare responses and intra-epidermal nerve fibre counts

doi: 10.1186/1471-2377-7-21

Figure Lengend Snippet: Intra-epidermal fibres for both the nerve marker – (PGP 9.5; a, b) and capsaicin receptor (TRPV1; c, d) are reduced in patient's skin. Scale bar = 50 μm.

Article Snippet: After rehydration, the tissue sections were incubated overnight with primary antibodies to the structural nerve marker PGP 9.5 (Ultraclone Ltd, Isle of Wight, UK; 1/50,000; for immersion fixed tissue sections) or the heat and capsaicin receptor TRPV1 (GlaxoSmithKline, Harlow, UK; 1/5,000, for post-fixed tissue sections).

Techniques: Marker

Journal: BMC Neurology

Article Title: Use of the novel contact heat evoked potential stimulator (CHEPS) for the assessment of small fibre neuropathy: correlations with skin flare responses and intra-epidermal nerve fibre counts

doi: 10.1186/1471-2377-7-21

Figure Lengend Snippet: Overview of sensory small fibre tests and correlations. All data presented from the leg.

Article Snippet: After rehydration, the tissue sections were incubated overnight with primary antibodies to the structural nerve marker PGP 9.5 (Ultraclone Ltd, Isle of Wight, UK; 1/50,000; for immersion fixed tissue sections) or the heat and capsaicin receptor TRPV1 (GlaxoSmithKline, Harlow, UK; 1/5,000, for post-fixed tissue sections).

Techniques: Electrofocusing